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The 2 protein bands from forty kDa to 30 kDa ended up absent right after 24 h incubation at 45 °C. For the rest of the bands, several concentrations of all three proteases retained protein bands akin to their primary molar masses (Determine 6a,b). The existence of bands at small molecular body weight in fish roe before hydrolysis was slot maxwin unexpected. During the report by Ghelichi et al. [8], insignificant peaks of a relatively lower molecular pounds vary (three–14 kDa) had been observed in defatted carp roe homogenate, most likely resulting from an autolytic procedure developing in fish roe and/or perhaps the defatting process bringing about the formation of lower-molecular-pounds peptides.
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